Background: Managing minor ailments in community pharmacy is an evolving pharmacy service in developing countries. Defined scopes of practice for pharmacy staff are essential for the safe management of minor ailments. Limited research exists regarding the perceptions of Indonesian pharmacists' and pharmacy technicians' scopes of practice in providing minor ailments management services. Objective: To evaluate pharmacists' and pharmacy technicians' understanding of their scopes of practice, perceived competency and factors influencing the delivery of minor ailments services in Indonesian community pharmacies. Methods: Cross-sectional surveys were conducted during January-February 2020 of pharmacists and pharmacy technicians attending seminars conducted by relevant Indonesian Associations in Central Java, Indonesia. Percentage of common responses (PCR) described similarity of perceived scopes of practice for pharmacists and pharmacy technicians. Univariate and multivariate analyses identified associations of scopes of practice with pharmacy characteristics. Results: A total of 185 pharmacists and 142 pharmacy technicians participated. Pharmacy technicians performed minor ailment consultations, however, if considered beyond their scope of practice, they referred the patient to the pharmacist (T=120/142, 84.5%). Vaginal thrush, bacterial conjunctivitis, gastro-oesophageal reflux disease, and acute pain were minor ailments perceived only within a pharmacist's scope (PCR above 60%). Of 34 minor ailments, 11 showed PCR values between 40-60% overlapping pharmacists and pharmacy technicians perceived scopes of practice (allergy/rash, back pain, cold sores, dermatitis, diarrhoea, eczema, hayfever, haemorrhoids, rheumatism, sore throat, and superficial wounds). Back pain, cold sores, dermatitis, and sore throat associated pharmacists' scope of practice with years of practice experience (p-value<0.05). Pharmacy technicians perceived their scopes of practice to be wider than perceived by pharmacists. Conclusions: Discordance between pharmacists' and pharmacy technicians' perceived scopes of minor ailments management highlights the need for clearly defined scopes of practice for each professional group. Each professional group must practise within their competence to ensure safe pharmacy practices.

O objetivo deste trabalho foi estudar a resposta da soja, com inoculação de Bradyrhizobium japonicum (estirpe CB1809, Semia 586) ou B. elkanii (estirpe 29W, Semia 5019), ao estresse hídrico. Plantas com inoculação da estirpe CB1809 produziram maior fração de raízes (19%) do que aquelas com inoculação de 29W (14,6%). As plantas com inoculação de CB1809 produziram menos nódulos e acumularam mais nitrogênio do que aquelas com inoculação de 29W. Em geral, baixos teores de ureídos nos nódulos foram encontrados em plantas irrigadas, com inoculação de CB1809 ou 29W, mas esses valores aumentaram cinco vezes em plantas com CB1809, sob estresse hídrico. Os nódulos formados pela estirpe CB1809 produziram, principalmente, aspartato e glutamato, ao passo que aqueles formados pela estirpe 29W produziram mais glutamato, asparagina e alanina. Nos nódulos de plantas com inoculação de CB1809, o aspartato foi o aminoácido que apresentou maior acumulação (5 µmol g-1); em plantas sob estresse hídrico, esse aminoácido alcançou 26 µmol g-1; e não foi detectada asparagina. Os nódulos formados pela estirpe 29W acumularam 1 µmol g-1 de aspartato, em plantas com ou sem estresse. A asparagina foi o principal aminoácido encontrado nos nódulos de plantas irrigadas (6 µmol g-1); a quantidade desse aminoácido foi aumentada em seis vezes, quando as plantas foram submetidas ao estresse hídrico.

The objective of this work was to study the response to water stress of a drought sensitive soybean cultivar inoculated with Bradyrhizobium japonicum (strain CB1809, Semia 586) and B. elkanii (strain 29W, Semia 5019). CB1809 nodulated plants produced a significantly higher root fraction (19%) than 29W (14.6%). Plants inoculated with CB1809 produced less nodules and accumulated more nitrogen than those inoculated with 29W. In general, low amounts of ureides in nodules were found in watered plants inoculated with either CB1809 or 29W strains, but those levels were five-fold increased in stressed plants inoculated with CB1809. Nodules formed by strain CB1809 had aspartate and glutamate as major amino acids, while those formed by 29W had glutamate, asparagine and alanine. In nodules of plants inoculated with CB1809 aspartate showed the highest accumulation (5 µmol g-1); in stressed plants this amino acid reached a value of 26 µmol g-1, and asparagine was not detected. Nodules formed by the strain 29W accumulated 1 µmol g-1 of aspartate, whether plants were stressed or not. Asparagine was the major amino acid found in nodules from watered plants (6 µmol g-1) and the amount of this amino acid was six-fold increased when plants were water stressed.

O objetivo deste trabalho foi investigar o efeito do estresse hídrico na fixação biológica do N2 e na estrutura dos nódulos de feijão (Phaseolus vulgaris L.), nas cultivares Carioca e EMGOPA-201. As plantas foram colhidas após cinco e oito dias de estresse hídrico. A cultivar Carioca apresentou menor peso seco de nódulos que o controle (plantas irrigadas). O peso da parte aérea seca da cultivar Carioca foi menor aos cinco dias de estresse hídrico e não diferiu do controle, após oito dias de estresse. As duas cultivares tiveram menor atividade da nitrogenase após cinco e oito dias de estresse hídrico e apresentaram danos na membrana peribacteróide após oito dias de estresse. A cultivar Carioca foi mais afetada do que a EMGOPA-201, com vacuolação das células e perda da membrana peribacteróide após cinco dias de estresse; aos oito dias, houve degradação das células do citoplasma, senescência e liberação dos bacteróides nos espaços intercelulares. A intensidade de glicoproteínas nos espaços intercelulares do córtex, marcada com os anticorpos MAC 236/265, foi diferente nas duas cultivares de feijão.

The aim of this work was to investigate the effect of water stress on N2 fixation and nodule structure of two common bean (Phaseolus vulgaris L.) cultivars Carioca and EMGOPA-201. Plants were harvested after five and eight days of water stress. Carioca had lower nodule dry weight on both water stress periods; shoot dry weight was lower at five days water stress and did not differ from control after eight days stress. Both cultivars had lower nitrogenase activity than control after five and eight days water stress. For both cultivars, after eight days stress bacteroid membranes were damaged. Carioca presented more pronounced damage to infected tissue, with host cell vacuolation and loss of the peribacteroid membrane at five days after stress; at eight days after stress, there was degradation of cytoplasm host cells and senescence of bacteroids, with their release into intercellular spaces. Intensity of immunogold-labeling of intercellular cortical glycoprotein with the monoclonal antibodies MAC 236/265 was different for both cultivars.